The variable region can be amplified using primers S1 and S8. This produces a product of ~1200 bp.
Reaction conditions: 30 cycles of 94°C for 60s, 55°C for 60s, 72°C for 90s
Sequence using primers S12 and S15.
Further details can be found in Thompson et al. 2003 Microbiology 149:1849-58.