FetA typing primers and protocol

PCR

The variable region can be amplified using primers S1 and S8.  This produces a product of ~1200 bp.

S1 CGGCGCAAGCGTATTCGG
S8 CGCGCCCAATTCGTAACCGTG

Reaction conditions: 30 cycles of 94°C for 60s, 55°C for 60s, 72°C for 90s

Sequencing

Sequence using primers S12 and S15.

S12 TTCAACTTCGACAGCCGCCTT
S15 TTGCAGCGCGTCRTACAGGCG

Further details can be found in Thompson et al. 2003 Microbiology 149:1849-58.