Primers used for MLST of Dichelobacter nodosus

 

Loci

Alleles

Primer 1 (5’-3’)

Primer 2 (5’-3’)

Tm °C

dtdA

19

CGGTCAAAATTGAACAAGGAACA

GATTCAGCGACGTTTGCTCG

60

folK

23

ATACGAAACCGCGCAAATCG

GGTGCGTCCGTTATTGACGA

60

rlmH

21

TTGCTGCCGTTATTCCGACT

CAACCAGCTGCTTTTCGATCC

60

rplI

20

CATTTGCCGGAATCGCTGTA

CAATTAGCAACCGCCGTCAA

60

tsaE

13

TTATCGTTTCGGCAACGCAC

ACCGCACAACAACACATCAC

60

dcd

20

CACGTTGGCTATCGTTTGGC

ATCGCGTTCTGTTCTGGTCT

60

recR

19

AACGCAACGAGAAATGAGCG

TAAAAACATCGCGCGGCAAA

60