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Primers - Lactococcal bacteriophage MLST Databases

Genes

The MLST scheme for the lactococcal phages of the 936-group uses internal fragments of the following five house-keeping genes:

PCR Amplification

Amplification primers were designed by M. Moisan at the Département de biochimie, de microbiologie et de bio-informatique, Université Laval, Québec, Canada.

The primer pairs we use for the PCR amplification of internal fragments of these genes are:

terL-F   5-GATGATTTTAGGCGGACAAT-3
terL-R   5-AGAACTTATTCTGTAACGCTG-3
mcp-F    5-GCAAAACTTGCTGAAAATGG-3
mcp-R    5-CTCATCTAACAAGGCTTTACG-3
mtp-F    5-TARCTGGTTTAGTATCRGTTGG-3
mtp-R    5-ACTGAWTCTGTTTCTGATTCTT-3
tmp-F    5-AAYTTACAAACGCAGTTGG-3
tmp-R    5-CAAAYGCTTGGTTAGCTTTTA-3
lys-F    5-AACAAACGGAGGATAAAAAAG-3
lys-R    5-AGTTCTGYRATAAARTATGAGC-3

Sequencing

We use the same primer pairs for sequencing.

Citing the database

The preferred format for citing this website in publications is:

This publication made use of the bacteriophage MLST website (https://pubmlst.org/ bacteriophages/) sited at the University of Oxford (Jolley et al. Wellcome Open Res 2018, 3:124 [version 1; referees: 2 approved]). The development of this site has been funded by the Wellcome Trust.

Status

Sequence database
Sequences: 702
Profiles (MLST): 221
Last updated: 2018-04-18

Isolate database
Isolates: 624
Last updated: 2018-01-16