| Gene | Primer sets a (5’ - 3’) |
Tm (°C) |
Amplicon size (bp) |
Gene fragment b size (bp) |
|---|---|---|---|---|
| nusA | P1 GCCTGCAAGARCCCGACAAG | 59 | 795 | 355 |
| P2 GTCCATSGCGTGCTTGTCTTC | ||||
| rpoB | P1 TGCCMTGGAACGGYTACAAC | 57 | 791 | 413 |
| P2 GGCCAG RTASACCTTGATCATCTT | ||||
| eno | P1 ATGCCCGTGCCSATGATGAA | 57 | 613 | 214 |
| P2 TCAGGGTGCCGATCTGGTTG | ||||
| gltB | P1 TGCAACCGGGCAAGATGTT | 57 | 685 | 241 |
| P2 TCGGACACGATCAGGATGTT | ||||
| lepA | P1 CTAYAACCTGAACCTGATCGACAC | 57 | 524 | 347 |
| P2 GCGACTTSGGCGTGAACAC | ||||
| nuoL | P1 CATGCACCAYRACCAGGACAT | 56 | 880 | 230 |
| P2 CGCGAACGCGTAGTGATAGATG | ||||
| nrdA | P1 GAACTGGATTCCCGACCTGTTC | 56 | 954 | 449 |
| P2 TTCGATTTGACGTACAAGTTCTGG |