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MLST of Streptococcus zooepidemicus

Genes

Internal fragments of the following genes are used:

Amplification

Genes were amplified by PCR using the following primer pairs:

LocusForwardReverse
arcCarcC1, 5'-AGC CAT CTA CCG ACT AAC AC-3'arcC4, 5'-TCT GAA AGG GTT TGG CTA GC-3'
nrdEnrdE1, 5'-TTC TCC TTC AGG TGA CAG ATG-3'nrdE4, 5'-AGA CTA GGC GTT TGA ACC TG-3'
proSproS1, 5'-TTG GTT GGA AAT GAC CAG ATC-3'proS4, 5'-CCT GAT CCT TGA CAT TAA CGG-3'
spispi1, 5'-CTT AGA GCA TTG CGC TAA GC-3'spi4, 5'-TTG CCT GCT ATC TAG GGA AG-3'
tdktdk1, 5'-GAA TTC AGG AAA GAC CAT TG-3'tdk4, 5'-TAA TGC TTG CGA CAA ACT GG-3'
tpitpi1, 5'-GGC AGT AGT AAG CAA ATT ACC-3'tpi4, 5'-AGC AAG GCA AGG AAG CTA TC-3'
yqiLyqiL1, 5'-CCA CAT GGG AAT TAC AGC AG-3'yqiL4, 5'-TCA ATA CAG ACG TCC CTT GAC C-3'

Sequencing

Sequencing was performed using the following primer pairs:

LocusForwardReverse
arcCarcC2, 5'-TGT CGC ACT TGG AGG AAA TG-3'arcC3, 5'-CAC CAC AAC ACC AGA ATC AAC-3'
nrdEnrdE2, 5'-CGC TCT ATT AAT TCT GCC TTG C-3'nrdE3, 5'-GCA TAG GTT GCT CAT GAT GAT G-3'
proSproS2, 5'-GAC TTC TTA GAG CCA GCT AG-3'proS3, 5'-AAC GGA GCT AGC TCT TTA GG-3'
spispi2, 5'-CCT ACT TTT TGG ACT CTC ACG-3'spi3, 5'-GAT TTT ATT GAG TGG CCA AAA GCG-3'
tdktdk2, 5'-CTT GAA GGT AGC ACA CAA TTA TG-3'tdk3, 5'-GGT CTC ATT GCC ACC AAT TTG-3'
tpitpi2, 5'-GGT CAC TAC AAT TGA GGC TG-3'tpi3, 5'-TTC AGG CTT CAC AGA ACC AC-3'
yqiLyqiL2, 5'-AGT ACC GCA ACG TAA AGG TG-3'yqiL3, 5'-GCC TGA CGC TTT TCC ATC TC-3'

Citing the database

The preferred format for citing this website in publications is:

This publication made use of the Streptococcus zooepidemicus MLST website (https://pubmlst.org/ szooepidemicus/) sited at the University of Oxford (Jolley et al. Wellcome Open Res 2018, 3:124 [version 1; referees: 2 approved]). The development of this site has been funded by the Wellcome Trust.

Status

Sequence database
Sequences: 698
Profiles (MLST): 394
Last updated: 2019-09-04

Isolate database
Isolates: 1,487
Last updated: 2019-09-04