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MLST of Acinetobacter baumannii complex (Oxford scheme)

Genes

The Acinetobacter baumannii complex MLST scheme uses fragments of the following seven house-keeping genes:

Citrate synthase (gltA)
DNA gyrase subunit B (gyrB)
Glucose dehydrogenase B (gdhB)
Homologous recombination factor (recA)
60-kDa chaperonin (cpn60)
Glucose-6-phosphate isomerase (gpi)
RNA polymerase sigma factor(rpoD)

Primers

Locus Primer Sequences Amplicon size (bp) Usage
gltA Citrato F1 AAT TTA CAG TGG CAC ATT AGG TCC C 722 amp/seq
Citrato R12 GCA GAG ATA CCA GCA GAG ATA CAC G amp/seq
gyrB gyrB_F TGA AGG CGG CTT ATC TGA GT 594 amp/seq
gyrB_R GCT GGG TCT TTT TCC TGA CA amp/seq
gdhB GDHB 1F GCT ACT TTT ATG CAA CAG AGC C 774 amp
GDH SEC F ACC ACA TGC TTT GTT ATG seq
GDHB 775R GTT GAG TTG GCG TAT GTT GTG C amp
GDH SEC R GTT GGC GTA TGT TGT GC seq
recA RA1 CCT GAA TCT TCY GGT AAA AC 425 amp/seq
RA2 GTT TCT GGG CTG CCA AAC ATT AC amp/seq
cpn60 cpn60_F GGT GCT CAA CTT GTT CGT GA 640 amp/seq
cpn60_R CAC CGA AAC CAG GAG CTT TA amp/seq
gpi gpi_F GAA ATT TCC GGA GCT CAC AA 456 amp/seq
gpi_R TCA GGA GCA ATA CCC CAC TC amp/seq
rpoD rpoD-F ACC CGT GAA GGT GAA ATC AG 672 amp/seq
rpoD-R TTC AGC TGG AGC TTT AGC AAT amp/seq

Citing the database

The preferred format for citing this website in publications is:

This publication made use of the Acinetobacter baumannii MLST website (https://pubmlst.org/ abaumannii/) sited at the University of Oxford (Jolley et al. Wellcome Open Res 2018, 3:124 [version 1; referees: 2 approved]). The development of this site has been funded by the Wellcome Trust.

Status

Sequence database
Sequences: 2,766
Profiles:
MLST (Oxford): 2,013
MLST (Pasteur): 1,385

Isolate database
Isolates: 4,374
Last updated: 2019-10-17