MLST of Streptococcus zooepidemicus
Genes
Internal fragments of the following genes are used:
- carbamate kinase (arcC)
- ribonucleoside-diphosphate reductase (nrdE)
- prolyl-tRNA synthetase (proS)
- signal peptidase I (spi)
- thymidylate kinase (tdk)
- triosephosphate isomerase (tpi)
- acetyl-CoA acetyltransferase (yqiL)
Amplification
Genes were amplified by PCR using the following primer pairs:
| Locus | Forward | Reverse |
|---|---|---|
| arcC | arcC1, 5'-AGC CAT CTA CCG ACT AAC AC-3' | arcC4, 5'-TCT GAA AGG GTT TGG CTA GC-3' |
| nrdE | nrdE1, 5'-TTC TCC TTC AGG TGA CAG ATG-3' | nrdE4, 5'-AGA CTA GGC GTT TGA ACC TG-3' |
| proS | proS1, 5'-TTG GTT GGA AAT GAC CAG ATC-3' | proS4, 5'-CCT GAT CCT TGA CAT TAA CGG-3' |
| spi | spi1, 5'-CTT AGA GCA TTG CGC TAA GC-3' | spi4, 5'-TTG CCT GCT ATC TAG GGA AG-3' |
| tdk | tdk1, 5'-GAA TTC AGG AAA GAC CAT TG-3' | tdk4, 5'-TAA TGC TTG CGA CAA ACT GG-3' |
| tpi | tpi1, 5'-GGC AGT AGT AAG CAA ATT ACC-3' | tpi4, 5'-AGC AAG GCA AGG AAG CTA TC-3' |
| yqiL | yqiL1, 5'-CCA CAT GGG AAT TAC AGC AG-3' | yqiL4, 5'-TCA ATA CAG ACG TCC CTT GAC C-3' |
Sequencing
Sequencing was performed using the following primer pairs:
| Locus | Forward | Reverse |
|---|---|---|
| arcC | arcC2, 5'-TGT CGC ACT TGG AGG AAA TG-3' | arcC3, 5'-CAC CAC AAC ACC AGA ATC AAC-3' |
| nrdE | nrdE2, 5'-CGC TCT ATT AAT TCT GCC TTG C-3' | nrdE3, 5'-GCA TAG GTT GCT CAT GAT GAT G-3' |
| proS | proS2, 5'-GAC TTC TTA GAG CCA GCT AG-3' | proS3, 5'-AAC GGA GCT AGC TCT TTA GG-3' |
| spi | spi2, 5'-CCT ACT TTT TGG ACT CTC ACG-3' | spi3, 5'-GAT TTT ATT GAG TGG CCA AAA GCG-3' |
| tdk | tdk2, 5'-CTT GAA GGT AGC ACA CAA TTA TG-3' | tdk3, 5'-GGT CTC ATT GCC ACC AAT TTG-3' |
| tpi | tpi2, 5'-GGT CAC TAC AAT TGA GGC TG-3' | tpi3, 5'-TTC AGG CTT CAC AGA ACC AC-3' |
| yqiL | yqiL2, 5'-AGT ACC GCA ACG TAA AGG TG-3' | yqiL3, 5'-GCC TGA CGC TTT TCC ATC TC-3' |