START2 - Load allele sequences

Allele sequences can be loaded by selecting 'File → Open or Enter → Allele sequences'. You will be presented with a dialog window, allowing you to choose which sequences to load.

If you have defined a MLST scheme, the defined loci will appear in the list, along with the path to the sequence file (either on a local drive or on a network resource) if entered. You can modify the path by double-clicking on the path cell for the particular locus and entering a value.

START2 also allows you to analyse sequence files that do not form part of a MLST scheme. These can be set up by entering the name of a locus in the text box and clicking the 'Add new locus' button.

The locus will then appear in the list. Double-click on the path and enter the location of the FASTA file (either on a local drive or on a network resource).

Non-MLST loci can be removed from the list by highlighting the specific row and clicking the 'Delete locus' button.

The sequence list will be remembered the next time you run START2.

You can choose which sequences to load from the list. When you have made your selection, press 'Load' to load them into memory.