Primers used for MLST of Streptococcus agalactiae
Internal fragments (400-500 bp) of the alcohol dehydrogenase (adhP), phenylalanyl tRNA synthetase (PheS), glutamine transporter protein (atr), glutamine synthetase (glnA), serine dehydratase (sdhA), glucose kinase (glcK) and transketolase (tkt) genes are sequenced.
Oligonucleotide primers for GBS MLST
| Locus | Forward (5' to 3') | Reverse (5' to 3') | Amplicon size (bp) |
|---|---|---|---|
| adhP amplification | GTTGGTCATGGTGAAGCACT | ACTGTACCTCCAGCACGAAC | 672 |
| sequencing | GGTGTGTGCCATACTGATTT | ACAGCAGTCACAACCACTCC | 498 |
| pheS amplification | GATTAAGGAGTAGTGGCACG | TTGAGATCGCCCATTGAAAT | 723 |
| sequencing | ATATCAACTCAAGAAAAGCT | TGATGGAATTGATGGCTATG | 501 |
| atr amplification | CGATTCTCTCAGCTTTGTTA | AAGAAATCTCTTGTGCGGAT | 627 |
| sequencing | ATGGTTGAGCCAATTATTTC | CCTTGCTCAACAATAATGCC | 501 |
| glnA amplification | CCGGCTACAGATGAACAATT | CTGATAATTGCCATTCCACG | 589 |
| sequencing | AATAAAGCAATGTTTGATGG | GCATTGTTCCCTTCATTATC | 498 |
| sdhA amplification | AGAGCAAGCTAATAGCCAAC | ATATCAGCAGCAACAAGTGC | 646 |
| sequencing | AACATAGCAGAGCTCATGAT | GGGACTTCAACTAAACCTGC | 519 |
| glcK amplification | CTCGGAGGAACGACCATTAA | CTTGTAACAGTATCACCGTT | 607 |
| sequencing | GGTATCTTGACGCTTGAGGG | ATCGCTGCTTTAATGGCAGA | 459 |
| tkt amplification | CCAGGCTTTGATTTAGTTGA | AATAGCTTGTTGGCTTGAAA | 859 |
| sequencing | ACACTTCATGGTGATGGTTG | TGACCTAGGTCATGAGCTTT | 480 |